PDE1C Assay Kit, BioAssay(TM)

Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE1C is a calmodulin-dependent PDE that is expressed principally in human myocardium.\n\nIntended Use:\nThe PDE1C Assay Kit is designed for identification of PDE1C inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE1C to the binding agent.\n\nPhosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled?cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide?product. This increases the size of the nucleotide relative to unreacted cyclic?monophosphate. In the polarization assay, dye molecules with absorption transition?vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes?attached to the rapidly-rotating cyclic monophosphates will obtain random orientations?and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead?complexes will not have time to reorient and therefore will emit highly polarized light.\n\nTest Principle:\nThe PDE1C inhibitor screening assay kit comes in a convenient 96-well format, including purified PDE1C enzyme, fluorescently labeled PDE1C substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE1C Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE1C reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE1C for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.\n\nKit Components:\nNUNC microtiter plate, 1x96wells\nRecombinant enzyme, 1x1ug\nFAM-Cyclic-3

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