PDE3B, Recombinant, Human, GST-Tag (Phosphodiesterase 3B, cGMP-inhibited, CGIP1, cGIPDE1, CGIPDE1, CGI-PDE B, cGMP-inhibited 3',5'-cyclic Phosphodiesterase B, Cyclic GMP-inhibited Phosphodiesterase B, HcGIP1)

Supplier:

United States Biological

Cat no: P4072-02R

PDE3B, Recombinant, Human, GST-Tag (Phosphodiesterase 3B, cGMP-inhibited, CGIP1, cGIPDE1, CGIPDE1, CGI-PDE B, cGMP-inhibited 3',5'-cyclic Phosphodiesterase B, Cyclic GMP-inhibited Phosphodiesterase B, HcGIP1)

Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-PDE11) based on their kinetic properties and inhibition to various pharmacological agents. The members of the PDE3 families are known as cGMP inhibited PDEs. The PDE3 enzymes bind both cGMP and cAMP with different affinities (cAMP 4-10-fold than cGMP). The phosphodiesterase type-3 (PDE3) family is comprised of 2 genes (PDE3A and PDE3B). PDE3A is a 145kD protein and is expressed in various tissues (myocardium, vascular and non vascular smooth muscles, megakaryocytes and a subset of neurons (Snyder 1999; Maecci et al., 1992). PDE3B is 1112aa long and has two splice variants which are expressed in adipocyte tissue, hepatocyte, kidney, spermatocytes and embryonic neuroepithelium (Reinhatdt et. al., 1995). Both PDE3A and PDE3B are therapeutic targets for the treatment of obesity as inhibition of PDE3 increase the lipolytic activity and alter the insulin secretion and its response. PDE3 inhibitors also promote thermogenic and cardiotonic effects in animals.\n\nSource: \nRecombinant human PDE3B, aa592-end, with N-terminal GST tag, expressed in an Baculovirus infected Sf9 cell expression system. \n\nMolecular Weight: 86kD\n\nSpecific Activity:\nLot-specific. Sample lot value: 1270pmol/min/ug\n\nUnit Definition:\n1 unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5' AMP per min at 30 degrees C in a reaction buffer. \n\nAssay Conditions: \n10mM Tris-HCl, pH7.4, 10mM MgCl2, 1mM MnCl2, 200uM cAMP, 2.5kU 5' nucleotidase, 37 degrees C, 20min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. \n\nApplication: \nUseful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. \n\nRecommended Dilution: \nOptimal dilution determined by the researcher.\n\nStorage and Stability:\nStore at -70 degrees C. Stable for at least 6 months. Avoid freeze-thaw cycles. or maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4072-02R

Size

10ug

Form

Supplied as a liquid in 40mM Tris, pH 8.0, 110mM NaCl, 2.2mM KCl, 3mM DTT, 0.04% \nTween-20, 20% glycerol

Purity

(same/more than)36%

References

1. Raymond, D.R. et al., Cell. Signal. 19 (12), 2507-2518 (2007). 2. Palmer, D. et al., J. Biol. Chem. 282 (13), 9411-9419 (2007).

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

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