PDE4D3 TR-FRET Assay Kit, BioAssay(TM)

Supplier:

United States Biological

Cat no: 137344

PDE4D3 TR-FRET Assay Kit, BioAssay(TM)

Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE4D is a regulator of airway smooth-muscle contractility, and has been identified as a potential risk predictor for ischemic stroke. Additionally, PDE4D has been associated with asthma pathophysiology and bone formation. The PDE4D gene encodes at least 9 different isoforms.\n\nIntended Use:\nThe TR-FRET PDE4D3 Assay Kit is designed for identification of inhibitors of PDE4D3 using TR-FRET (Time Resolved Fluorescence Resonance Energy Transfer) technology. The assay is based on the binding of phosphate groups from FAM-labeled nucleotide monophosphates generated by PDE4D3 and from terbium donors to the nanoparticle binding agent. The binding will result in energy transfer from terbium to FAM and the fluorescent intensity change can be measured using fluorescence reader. \n\nTest Principle:\nThe TR-FRET PDE4D3 Assay Kit comes in a convenient 96-well format, with purified PDE4D3 enzyme, fluorescently labeled PDE4D3 substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. Using this kit, only two simple steps on a microtiter plate are required for the PDE4D3 activity assay. First, the fluorescent-labeled cAMP is incubated with a sample containing PDE4D3 for 1 hour. Second, a binding agent and a terbium donor are added to the reaction mix and incubated for 1 hour. Then, fluorescence intensity can be measured using a fluorescence reader.\n\nKit Components:\nNUNC microtiter plate, 1x96wells\nRecombinant enzyme, 1x1ug\nFAM-Cyclic-3

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

137344

Size

96Tests

References

1. Bender, A., et al., Pharmacol. Rev. 58: 488-520 (2006).

Read more on Supplier website

SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

Website:

More from
United States Biological

All suplier products

United States Biological

EBI3, Control Peptide (Interleukin-27 Subunit beta, IL-27 Subunit beta, IL-27B, Epstein-Barr Virus-induced Gene 3 Protein, EBV-induced Gene 3 Protein, IL27B)

Applications

ELISA

United States Biological

RNASEH2A, Control Peptide (Ribonuclease H2 Subunit A, RNase H2 Subunit A, Ribonuclease HI Subunit A, Ribonuclease HI Large Subunit, RNase HI Large Subunit, RNase H(35), Aicardi-Goutieres Syndrome 4 Protein, AGS4, RNASEHI, RNHIA)

Applications

ELISA

United States Biological

CRMP1, Control Peptide (Dihydropyrimidinase-related Protein 1, DRP-1, Collapsin Response Mediator Protein 1, CRMP-1, Unc-33-like Phosphoprotein 3, ULIP-3, DPYSL1, ULIP3)

Applications

ELISA

United States Biological

IL16, Control Peptide (IL-16, Pro-interleukin-16, Lymphocyte Chemoattractant Factor, LCF)

Applications

ELISA

United States Biological

OCC1, Control Peptide (Overexpressed in Colon Carcinoma 1 Protein, OCC-1, AGD3, C12orf75, MGC148100, MGC149157)

Applications

ELISA

United States Biological

KLRA1, Control Peptide (Killer Cell Lectin-Like Receptor Subfamily A, Member 1, KLRA#, LY49L, Ly-49L, Ly49, MGC126520, MGC126522)

Applications

ELISA

United States Biological

SLC39A10, Control Peptide (Zinc Transporter ZIP10, Solute Carrier Family 39 Member 10, Zrt- and Irt-like Protein 10, ZIP-10, KIAA1265, ZIP10)

Applications

ELISA

United States Biological

Klra3, Control Peptide (Killer Cell Lectin-like Receptor 3, 5E6, Lymphocyte Antigen 49c, Ly-49c, Nk2.1, T-cell Surface Glycoprotein Ly-49C, Ly-49c, Ly49C, MGC123910)

Applications

ELISA

Advertisehere

BMA Biomedicals Horizontal banner

Latest promotion FROM:

BMA Biomedicals

Latest promotions

Explore all promotions

B-Bridge International, Inc.

DNA Cleanup in 7 Minutes, 10% OFF

Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...

BD (Becton, Dickinson and Company)

Analyze your cells with more colors at lower prices!

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

Europa Bioproducts Ltd

20% discount on Bovine Serum Albumin!

As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....

Cecil Instruments Limited

Cecil Spectrophotometer Enhancements for Free!

It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...

Advanced Targeting Systems, Inc.

Secondary Conjugates - Save 20%

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

Cayman Chemical Company

Cayman Challenge

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

SDIX

Custom Polyclonal Antibodies

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

Innovative Research, Inc

Premium US Origin Fetal Bovine Serum for only $95 per 500ml!

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

Cell Sciences, Inc.

Recombinant Proteins

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Bibby Scientific

73 series spectrophotometer

Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...

Everest Biotech Ltd

Custom antibody for $300

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...

Top suppliers

Explore all suppliers

United States Biological

230753 products

Carl Zeiss Microscopy

27 products

Promega Corporation

11 products

Panasonic Healthcare Company

5 products

Life Technologies

1 products

Nikon Instruments Europe

11 products

Olympus Europa Holding GmbH

3 products

Leica Microsystems, Inc.

10 products

GE Healthcare Life Sciences

2 products

Tecan Trading AG

19 products

Beckman Coulter, Inc.

1 products

AB SCIEX

3 products

BD (Becton, Dickinson and Company)

1 products

RANDOX TOXICOLOGY

5 products

Randox Food Diagnostics

6 products