PDE5A Assay Kit, BioAssay(TM)

Supplier:

United States Biological

Cat no: 137346

PDE5A Assay Kit, BioAssay(TM)

Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE5A, also known as cGMP-inhibited phosphodiesterase, has been implicated in cardiovascular function and fertility.\n\nIntended Use:\nThe PDE5A Assay Kit is designed for identification of PDE5A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE5A to the binding agent. Phosphodiesterases catalyze the hydrolysis of the phosphodiester bond in dye-labeled cyclic monophosphates. Beads selectively bind the phosphate group in the nucleotide product. This increases the size of the nucleotide relative to unreacted cyclic monophosphate. In the polarization assay, dye molecules with absorption transition vectors parallel to the linearly-polarized excitation light are selectively excited. Dyes attached to the rapidly-rotating cyclic monophosphates will obtain random orientations and emit light with low polarization. Dyes attached to the slowly-rotating nucleotide-bead complexes will not have time to reorient and therefore will emit highly polarized light.\n\nTest Principle:\nThe PDE5A inhibitor screening assay kit comes in a convenient 96-well format, with purified PDE5A enzyme, fluorescently labeled PDE5 substrate (cGMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE5A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE5A reactions. First, the fluorescently labeled cGMP is incubated with PDE5A for 1 hour. Second, the binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.\n\nKit Components:\nNUNC microtiter plate, 1x96wells\nRecombinant enzyme, 1x1ug\nFAM-Cyclic-3

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SPECIFICATIONS

Catalog Number

137346

Size

96Tests

References

1. Maurice, D.H., Front. Biosci. 10: 1221-1228 (2005).

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SUPPLIER INFO

United States Biological

Address:

4 Technology Way, Salem, MA 01970, US

Phone:

+1 800 520 3011

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