PDGF-AB BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human PDGF-AB in cell culture supernates, serum and plasma (heparin, EDTA).\n\nThe platelet-derived growth factor (PDGF) is a mitogen derived from human platelets consisting of two related polypeptides termed A and B chains.1 The genes for PDGF A chain, B chain/c-sis, and the PDGF receptor are expressed in human malignant glioma cell lines.2 Normal human endothelial cells in culture express the B chain of PDGF, and that endothelial-derived PDGF B chain is synthesized as a predicted precursor polypeptide of Mr 27,281.3 The entire B chain of PDGF is highly (96%) homologous to a portion of p28sis, the transforming protein of simian sarcoma virus (SSV). It has been suggested that p28sis exerts its transforming potential by mimicking the growth promoting activity of PDGF and stimulating the cell in an autocrine manner.1 PDGF A-chain precursor polypeptide is assigned to the proximal long arm of chromosome 7, band q11.23.4 The human homolog (PDGF B-chain/c-sis) of the transforming gene of simian sarcoma virus is assigned to chromosome 22.5 The standard product used in this kit is recombinant human PDGF-AB with the molecular mass of 27kD.\n\nAssay Range:\n31.2pg/ml-2000pg/ml\n\nSensitivity:\n< 2 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP01127, P04085\n\nEntrez Gene ID:\n5155, 5154\n\nKit Components:\n96-well plate pre-coated with anti-PDGF-AB, Human\nLyophilized recombinant PDGF-AB, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144006

Size

96Tests

References

1. Kelly, J. D.; Raines, E. W.; Ross, R.; Murray, M. J. The B chain of PDGF alone is sufficient for mitogenesis. EMBO J. 4: 3399-3405, 1985. \n2. Hermansson, M.; Nister, M.; Betsholtz, C.; Heldin, C.-H.; Westermark, B.; Funa, K. Endothelial cell hyperplasia in human glioblastoma: coexpression of mRNA for platelet-derived growth factor (PDGF) B chain and PDGF receptor suggests autocrine growth stimulation. Proc. Nat. Acad. Sci. 85: 7748-7752, 1988. \n3. Collins, T.; Ginsburg, D.; Boss, J. M.; Orkin, S. H.; Pober, J. S. Cultured human endothelial cells express platelet-derived growth factor B chain: cDNA cloning and structural analysis. Nature 316: 748-750, 1985. \n4. Stenman, G.; Rorsman, F.; Betsholtz, C. Sublocalization of the human PDGF A-chain gene to chromosome 7, band q11.23, by in situ hybridization. Exp. Cell Res. 178: 180-184, 1988. \n5. Dalla-Favera, R.; Gallo, R. C.; Giallongo, A.; Croce, C. Chromosomal localization of the human homolog (c-sis) of the simian sarcoma virus onc gene. Science 218: 686-688, 1982.\n