Home  >  Products  >  PE anti-mouse/rat TNF-alpha
PE anti-mouse/rat TNF-alpha

PE anti-mouse/rat TNF-alpha

Cat no: 506104


Supplier: BioLegend
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
TNF-alpha is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-alpha. Monomeric mouse TNF-alpha is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-alpha forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-alpha has also been described. TNF-alpha binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The TN3-19.12 antibody reacts with mouse, rat, and rabbit tumor-necrosis factor-alpha (TNF-alpha) proteins. The TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-alpha.
Catalogue number: 506104
Reactivities: Mouse, Rat
Hosts: Hamster
Applications: Flow Cytometry, Immunocytochemistry
Size: 100 microg
Clone: TN3-19.12
Gene: 21926,24835
Dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometricanalysis. For immunofluorescent staining, the suggested use of this reagent is ? 0.25 microg per million cells in 100 microl volume or 100 microl of whole blood. It isrecommended that the reagent be titrated for optimalperformance for each application.
Storage buffer: The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.
Concentration: 0.2 mg/ml
Antigen: E. coli-expressed, recombinant mouse TNF-alpha
Isotype: Armenian Hamster IgG
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Tumor necrosis factor-alpha, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave