PECAM-1/CD31 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human PECAM-1/CD31 in cell culture supernates, serum and plasma (heparin, EDTA).\n\nHuman platelet/endothelial cell adhesion molecule-1 (PECAM1), an important member of the immunoglobulin gene superfamily, is widely distributed on cells of the vascular system and mediates cellular interactions through both homophilic and heterophilic adhesive mechanisms. The function of PECAM1 in vitro has begun to be understood, but its function in vivo is yet to be established.1 The PECAM1 locus is on the long arm of chromosome 17, in the region q23-qter. To confirm this observation and obtain a more precise localization of the PECAM1 locus, fluorescence in situ hybridization was conducted. Together our data allowed assignment of the PECAM1 locus to the region 17q23.2 To examine the functional role of PECAM-1 in regulating platelet-collagen interactions, 2 different approaches were applied using recombinant human PECAM-1-immunoglobulin chimeras and platelets derived from PECAM-1-deficient mice.3 PECAM-1, an integral membrane protein with an essential role in TEM, is found in this compartment and constitutively recycles evenly along endothelial cell borders.4 The standard used in this kit is the product of gene recombinant expression (extracellular part), consisting of 574 amino acids (Q28-K601) with the molecular weight of 64.5kD. After glycosylating, its molecular weight changes to 90-100kD.\n\nAssay Range:\n156pg/ml-10000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP16284\n\nEntrez Gene ID:\n5175\n\nKit Components:\n96-well plate pre-coated with anti-PECAM-1/CD31, Human\nLyophilized recombinant PECAM-1/CD31, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

144010

Size

96Tests

References

1. Xie, Y., Muller, W. A. Fluorescence in situ hybridization mapping of the mouse platelet endothelial cell adhesion molecule-1 (PECAM1) to mouse chromosome 6, region F3-G1. Genomics 37: 226-228, 1996.\n2. Gumina, R. J., Kirschbaum, N. E., Rao, P. N., vanTuinen, P., Newman, P. J. The human PECAM1 gene maps to 17q23. Genomics 34: 229-232, 1996. \n3. Jones, K. L., Hughan, S. C., Dopheide, S. M., Farndale, R. W., Jackson, S. P., Jackson, D. E. Platelet endothelial cell adhesion molecule-1 is a negative regulator of platelet-collagen interactions. Blood 98: 1456-1463, 2001.\n4. Mamdouh, Z., Chen, X., Pierini, L. M., Maxfield, F. R., Muller, W. A. Targeted recycling of PECAM from endothelial surface-connected compartments during diapedesis. Nature 421: 748-753, 2003.\n