PEDF, Recombinant, Human (Pigment Epithelial Derived Factor, SerpinF1, EPC-1)

Recombinant Human PEDF (Pigment epithelium-derived factor, SerpinF1, EPC-1) is a noninhibitory serpin with neurotrophic, anti- angiogenic, and anti-tumorigenic properties. It is a 50kD glycoprotein produced and secreted in many tissues throughout the body. A major component of the anti- angiogenic action of PEDF is the induction of apoptosis in proliferating endothelial cells. In addition, PEDF is able to inhibit the activity of angiogenic factors such as VEGF and FGF-2. The neuroprotective effects of PEDF are achieved through suppression of neuronal apoptosis induced by peroxide, glutamate, or other neurotoxins. \n\nThe recent identification of a lipase-linked cell membrane receptor for PEDF (PEDF-R) that binds to PEDF with high affinity (1) should facilitate further elucidation of the underlying mechanisms of this pluripotent serpin. To date, PEDF-R is the only signaling receptor known to be used by a serpin family member. The unique range of PEDF activities implicate it as a potential therapeutic \nagent for the treatment of vasculature related neurodegenerative diseases such as age-related macular degeneration (AMD) and proliferative diabetic retinopathy (PDR). PEDF also has the potential to be useful in the treatment of various angiogenesis-related diseases including a number of cancers. \n\nRecombinant PEDF is a 44.5kD non-glycosylated protein containing 400 amino acid residues. \n\nSource:\nRecombinant human PEDF (pigment epithelium-derived factor) expressed in E. coli cells..\n\nBiological Activity: Data Not Available\n\nAA Sequence: MQNPASPPEE GSPDPDSTGA LVEEEDPFFK VPVNKLAAAV \nSNFGYDLYRV RSSMSPTTNV LLSPLSVATA LSALSLGAEQ RTESIIHRAL YYDLISSPDI HGTYKELLDT VTAPQKNLKS ASRIVFEKKL RIKSSFVAPL EKSYGTRPRV LTGNPRLDLQ EINNWVQAQM KGKLARSTKE IPDEISILLL GVAHFKGQWV TKFDSRKTSL EDFYLDEERT VRVPMMSDPK AVLRYGLDSD LSCKIAQLPL TGSMSIIFFL PLKVTQNLTL IEESLTSEFI HDIDRELKTV QAVLTVPKLK LSYEGEVTKS LQEMKLQSLF DSPDFSKITG KPIKLTQVEH RAGFEWNEDG AGTTPSPGLQ \nPAHLTFPLDY HLNQPFIFVL RDTDTGALLF IGKILDPRGP \n\nEndotoxin: (same/less than)0.1ng/ug (1EU/ug). \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\n

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SPECIFICATIONS

Catalog Number

P3140-02A

Size

5ug

Form

Supplied as a lyophilized powder.

Purity

(same/more than) 90% by SDS-PAGE, HPLC

References

1. Notari, I. et al. J Biol Chem., 281:38022-38037. 2. Yabe, T., et al., (2001) J. Biol. Chem. 276:43,313-43,319. 3. Alberdi, E., et al., (1999) J. Biol. Chem. 274:31,605-31,612. 4. Stratikos, E., et al., (1996) Protein Science 5:2575-2582.