Protein sample identification generally only uses Trypsin for protein digestion, with peptide coverage identified around 60%. To obtain the complete sequence information of the target protein, MtoZ Biolabs will use 6 commonly used proteinases in protein identification (Trypsin, Chymotrypsin, Asp-N, Glu-C, Lys-C, and Lys-N) to digest and identify the target protein. By obtaining more fragmented peptide fragments and stitching them together, 100% of the protein sequence is determined.
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