Home  >  Products  >  PerCP anti-mouse CD43 Activation-Associated Glycoform
PerCP anti-mouse CD43 Activation-Associated Glycoform

PerCP anti-mouse CD43 Activation-Associated Glycoform

Cat no: 121222

Supplier: BioLegend
Star_fadedStar_fadedStar_fadedStar_fadedStar_faded
0 reviews | Write a Review Pencil
CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5+ B cells. It is not present on resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with the activation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared with desialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells and memory T cells.
Catalogue number: 121222
Reactivities: Mouse
Hosts: Rat
Applications: Flow Cytometry
Size: 100 microg
Clone: 1B11
Gene: 20737
Dilutions: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is ? 1.0 microg per 106 cells in 100 microl. It is recommended that the reagent be titrated for optimal performance for each application.* PerCP has a maximum absorption of 482 nm and 564 nm and a maximum emission of 675 nm.
Storage buffer: The antibody solution should be stored undiluted at 4C and protected from prolonged exposure to light. Do not freeze.
Concentration: 0.2 mg/ml
Antigen: Mouse WEHI 274.3 myeloid tumor cells
Isotype: Rat IgG2a, kappa
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Leukosialin, Ly-48, Sialophorin, gp115
See Full Product Listings >
Button_on Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave Button_off_biosave