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Perilipin (PERI, PLIN, Lipid Droplet-associated Protein)

Perilipin (PERI, PLIN, Lipid Droplet-associated Protein)

Cat no: P3340-98G

Supplier: United States Biological
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The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encdoneoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Applications: Suitable for use in Immunofluorescence, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence: 1ug/ml ELISA: 1:32,000 Western Blot: 0.01-0.03ug/ml, observed in human adipose lysates on ~55-60kD bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Catalogue number: P3340-98G
Reactivities: Human, Rat, Canine
Hosts: Goat
Applications: ELISA, Immunofluorescence, Western Blot
Size: 100ug
Form: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
P type: Pab
Purity: Purified by immunoaffinity chromatography.
References: 1. Wolins NE, Brasaemle DL, Bickel PE. A proposed model of fat packaging by exchangeable lipid droplet proteins. FEBS Lett. 2006 Oct 9;580(23):5484-91. Epub 2006 Sep 1.
Additional info: Recognizes human PLIN. Species sequence homology: Canine and rat.

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