Period Clock Protein 2, Mouse, Control Peptide (Per2, RIGUI)

A 23-aa peptide sequence within C-terminus of mouse Per2.\n\nSeveral endogenous factors have been linked to rhythmicity or circadian behavior of living organisms. In Drosophila, the genes period (dPer) and timeless (tim), and in Neurospora frequency (frq), have been proposed to be responsible for their circadian rhythm. Recently human and mouse genes encoding a basichelix-loop-helix (bHLH) and Per-ARNT-Sim (PAS)-domain with significant similarity to the Drosophila Period have been reported. The cDNA sequences of hPER and mPer1 (also named RIGUI) are predicted to encode for proteins of length 1290 and 1291 amino acids respectively. Homologues of mPer1 designated Per 2 (1257 aa) and Per3 (1113 aa) have also been cloned. Both Per1 and Per2 levels show circadian rhythm in the SCN and eyes. It has been suggested that mPer regulates neuronal activity in the SCN. Using genetic approach, a single mutation (A to T in the Clock gene affects circadian rhythmicity in mice. Clock has been mapped to chromosome 5. Mouse Clock encodes a transcription factor, a single polypeptide chain of 855 aa (predicted calculated mol wt ~97kD; pI 6.52; hClock, 846 aa). Clock is abundantly expressed in brain (SCN, pyrifom cortex, hippocampus) as well as in other tissues (eye, total brain, tested, ovaries, liver, heart, lung, and kidney). Although, Clock is constitutively expressed (not rhythmic) in the SCN, it may still be an important component of circadian machinery.

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SPECIFICATIONS

Catalog Number

P3342-20C

Size

50ug

Applications

ELISA, WB

Form

Supplied as a liquid in PBS, pH 7.2

Purity

Chromatographically purified

References

(1). Albretch U et al (1997) Cell 91, 1055-1064; Shearman LP et al (1997) Neuron 19, 1261-1269; Sakamoto K et al (1998) J Biol. Chem. 273, 27039-27342. (2) Nagase T eat al (1997) DNA Res. 4, 141-150\n(3) Field MD et al (2000) Neuron 25, 437; Bae K et al (2000) 30, 525-536; Nuesslein-Hildesheim B et al (2000) Eur. J. Neurol. 2856.\n

Additional Info

77% conserved in rat Per2. No significant homology is seen with human Per2 or Per1 or Per3. Antibody crossreactivity with Per2 from other species is not known. Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommed for ELISA, Dot Blot, or for antibody blocking experiments