Permeabilization Wash Buffer (10X)

Cat no: 421002

Supplier: BioLegend

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Description
Additional Information

Permeabilization Wash Buffer is useful for intracellular staining procedures, e.g., in preparation of cells for staining intracellular cytokines or other proteins. Permeabilization Wash Buffer is used to permeabilize cells following fixation with Fixation Buffer (Cat. No. 420801). It is supplied as a 10X solution and should be diluted in deionized water prior to use. Permeabilization Wash Buffer has been formulated to have minimal effects on cells, reduce non-specific staining and enhance the signal to noise ratio. It can be used for antibody dilutions and cell washing during intracellular staining.

Catalogue number:	421002
Applications:	Flow Cytometry, Immunocytochemistry, Immunohistochemistry
Size:	100 ml
Dilutions:	For use in permeabilization, dilute Permeabilization Wash Buffer (10X) to 1X in DI water. Resuspend fixed cells in diluted Permeabilization Wash Buffer and centrifuge at 350 xg for 5-10 minutes, and repeat the same process for twice. It is recommended that the reagent be titrated for optimal performance for each application. Please see "Intracellular Cytokine Staining Protocol" under "Support" on BioLegend's website.
Storage buffer:	This permeabilization wash buffer solution should be stored at 4C. Do not freeze.
Regulatory status:	RUO
Shipping temp:	Ambient RT
Storage temp:	4 C
Alternative names:	Perm/Wash, Permeabilization Buffer