Peroxide Assay Kit, BioAssay(TM), Colorimetric

Peroxide (e.g. hydrogen peroxide H2O2) is one of the key reactive oxygen species formed under oxidative stress conditions. High levels of peroxide formation have been linked to pathological conditions such as ageing, asthma, diabetes, atherosclerosis, cataract, inflammatory arthritis and neurodegenerative diseases.\n\nSimple, direct and automation-ready procedures for quantitative determination of peroxide find wide applications in research and drug discovery. Peroxide assay kit is designed to measure peroxide concentration in biological samples without any pretreatment. The improved method utilizes the chromogenic Fe3+-xylenol orange reaction, in which a purple complex is formed when Fe2+ provided in the reagent is oxidized to Fe3+ by peroxides present in the sample. The intensity of the color, measured at 540-610nm, is an accurate measure of the peroxide level in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.\n\nKey Features:\nSensitive and accurate. Enhanced color intensity using sorbitol.\nDetection range 0.2uM (7 ng/mL) to 30uM (1,020 ng/mL) H2O2 in 96-well plate assay.\nSimple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.\n\nApplications:\nDirect Assays: H2O2 in biological samples (e.g. serum, citrate-plasma, urine, cell lysate, culture medium).\nPharmacology: effects of drugs on peroxide metabolism.\n\nKit Contents: (250 tests in 96-well plates)\nReagent A: 1ml\nReagent B: 50ml\nStandard: 100ul 3% stabilized H2O2.\nKit shipped at room temperature.\nStorage conditions. The kit is shipped at room temperature. Store all reagents at 4 degrees C. Shelf life: 6 months after receipt.\nPrecautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.\n\nProcedures:\nSample Treatment: several chemicals are known to interfere and should be avoided in sample preparation. These include ascorbic acid, EDTA, heparin, DMSO (>0.02%), NP-40 (>0.6%), SDS (>0.12%), Tris (>8mM) and ethanol (>0.4%). Samples can be analyzed immediately after collection, or stored in aliquots at

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SPECIFICATIONS

Catalog Number

P3351-71

Size

1Kit

References

1. Chi Li Yu et al (2008). A novel caffeine dehydrogenase in pseudomonas sp. strain CBB1 oxidizes caffeine to trimethyluric acid. J. Bacteriol. 190(2): 772