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Peroxiredoxin 5 (PRDX5, ACR1, AOEB116, B116, PLP, PMP20, PRDX6, PRXV, SBBI10)

Cat no: P3352-34

Peroxiredoxin 5 (PRDX5, ACR1, AOEB116, B116, PLP, PMP20, PRDX6, PRXV, SBBI10)

Peroxiredoxin 5 (Prx-5; also known as AOEB166 and thioredoxin reductase) is a 22kD, \nwidely expressed mitochondrial antioxidant enzyme that belongs to the atypical 2-Cys class of the TSA/ahpC family of peroxiredoxins. The mouse precursor molecule is 210 amino acids (aa) in length and contains an N-terminal 48 aa mitochondrial targeting (signal) sequence and a 162 aa mature enzyme that shows an NES between aa 54-63, and a peroxisome targeting motif at aa 208-210. There are two catalytic cysteines, one at Cys96, and another at Cys200 of the precursor. Prx-5 is a monomer that is found in mitochondria, cytosol, nucleus and peroxisomes. It is known to reduce H 2O2 and alkyl hydroperoxides. Two potential splice forms are reported. One is 17kD in size and shows an alternative start site at Met49, while a second shows a 19 aa substitution for aa 83-102. Mouse Prx-5 is 78% and 90% aa identical to human and rat Prx-5, respectively.\n\nApplications:\nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile 40-50% glycerol, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P3352-34

Size

100ug

Applications

WB

Hosts

Goat

Reactivities

Hum, Mouse, Rat

Form

Supplied as lyophilized from a 0.2um sterile-filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS. If 0.1ml of PBS is used, the antibody concentration will be 1mg/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.\n

Isotype

IgG

Additional Info

Detects endogenous human, mouse and rat Peroxiredoxin 5 at ~22kD by Western blot.

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