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PERP, aa175-193 (PIGPC1, THW)

Cat no: P3353

PERP, aa175-193 (PIGPC1, THW)

The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including CHK2 (1), p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), PID/MTA2 (6), MTBP (7) and PERP (8), were identi?ed and their genes were cloned recently. PERP, also termed PIGPC1 and THW, is a plasma membrane protein (8-10). p53 binds to the promoter of PERP and transcriptionally activates PERP gene then the translated PERP protein mediates the p53 induced apoptosis (8). The expression of PERP causes cell death. PERP is a mediator of p53 induced apoptosis. PERP has sequence similarity to PMP-22/gas3 and is a new member of the PMP-22/gas3 family (8).\n\nApplications: Suitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution: Western Blot: 0.5-1ug/ml; Optimal dilutions to be determined by the researcher.\n\nPositive Control: A431 cell lysate\n\nStorage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P3353

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Pab

Purity

Purified

Isotype

IgG

References

1. Matsuoka S, Huang M, Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science. 1998;282:1893

Additional Info

Recognizes a 21kD band.

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