CXCL4 is a CXC chemokine that is expressed in megakaryocytes and stored in the alpha-granules of platelets. CXCL4 is chemotactic towards neutrophils and monocytes and has been shown to inhibit angiogenesis. Recombinant human CXCL4 is a 7.8kD protein containing 70aa, including the four highly conserved residues present in CXC chemokines.
Source:
Recombinant protein corresponding to human CXCL4, expressed in E. coli.
Biological Activity:
Determined by its ability to chemoattract human fibroblasts using a concentration range of 1-10ng/ml.
Endotoxin Level:
<0.1ng/ug (1EU/ug)
AA Sequence:
EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES
Storage and Stability:
Lyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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