Validated for a wide range of urine, fecal, serum and plasma samples
Robust results in 90 minutes
The 13,14,Dihydro-15-keto-Prostaglandin F-2alpha (PGFM) Enzyme Immunoassay kit measures PGFM present in non-invasively collected fecal and urine samples. The assay will also measure PGFM in plasma, serum and TCM samples. The kit has been developed for urine and extracted fecal samples and has been validated with Iberian lynx and other felid samples. Diluted samples are pipetted into a clear microtiter plate coated with an antibody. A PGFM-peroxidase conjugate is added to the wells. The binding reaction is initiated by the addition of a polyclonal antibody to PGFM. After an hour incubation the plate is washed and substrate is added. The substrate reacts with the bound PGFM-peroxidase conjugate. After a short incubation, the reaction is read at 450 nm wavelength.
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