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PGR, ID (PGR, NR3C3, Progesterone receptor)

Cat no: 136326

PGR, ID (PGR, NR3C3, Progesterone receptor)

The progesterone receptor, a NR3 Steroid Receptor, is induced in granulosa cells of preovulatory follicles in response to surges in luteinizing hormone. It is essential for ovulation and egg implantation. A decrease in expression of endometrial progesterone receptor results in infertility in women. This receptor mediates the progesterone-induced transcription of proteases including ADAMTS1 and cathepsin L. In breast cancer, progesterone receptors are associated with hormone dependence and prolonged survival. At least two alternatively spliced isoforms have been identified in human: hPR-A (94kD) is 164aa shorter than hPR-B (114kD). In most cell contexts, hPR-B functions as a transcriptional activator of progesterone-responsive genes, whereas hPR-A functions as a transcriptional inhibitor of all steroid hormone receptors. hPR-A and hPR-B have been shown to regulate different sets of genes in human breast cancer cells. Some of these PR-regulated genes are implicated in breast cancer.\n\nApplications: \nSuitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:100-1:500 \nELISA: 1:1000\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (Paraffin): 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

136326

Size

50ug

Applications

ELISA, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography

Isotype

IgG

Additional Info

Recognizes human PGR/Progesterone.

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