Phage Titration BioAssay(TM) ELISA Kit

Phage Titration ELISA; Kit allows fast titration of M13 particles by immunological recognition of protein III. Contains standard M13 titrated by electron microscopical method.\n\nThe phage display technology is a powerful and well-established tool for the investigation of protein-protein interactions and for the generation of human antibodies for in vitro diagnostics and in vivo therapy (Koch et al. 2000). Generally several rounds of selection on an immobilized antigen are required to enrich and isolate the binding phage. The need to determine phage titers before and after each round to monitor the panning process still represents a major time and material consuming factor within this procedure. Usually, the phages are titered by infecting E. coli with serial dilutions of phage, plating the bacteria on agar plates and counting the colony (cfu) or plaque forming units (pfu) after over-night incubation (Koch et al., 2000). To determine the relative number of phage particles, however, immunotitration by Phage Titration ELISA offers a fast, sensitive and reproducible alternative for titration of M13 bacteriophage preparations.\n\nReagent kit, 12 x 8-well strips.\nIncubation: 60/60 min.\nStandard range: 1 x 107-1 x 10e8 particles /ml\n\nKit Components:\nMicrotiter plate, 1x96 wells\nControl (purified M13 particles), 2x1vial\nConjugate (20x), 1x750ul\nWash buffer (20x), 1x20ml\nSubstrate TMB, 1x12ml\nStop solution, H2SO4, 1x13ml\n\nStorage and Stability:\nStore components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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