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PHD2 (PH2, Prolyl Hydroxylase Domain Containing Protein 2, HIF2PH2, HIF-Prolyl Hydroxylase 2, EGLN1, Egl Nine Homolog 1, C1orf12, Chromosome 1 Open Reading Frame 12, PNAS-118, PNAS-137, Hypoxia-inducible Factor Prolyl Hydroxylase 2, SM-20)

Cat no: P3374-03

PHD2 (PH2, Prolyl Hydroxylase Domain Containing Protein 2, HIF2PH2, HIF-Prolyl Hydroxylase 2, EGLN1, Egl Nine Homolog 1, C1orf12, Chromosome 1 Open Reading Frame 12, PNAS-118, PNAS-137, Hypoxia-inducible Factor Prolyl Hydroxylase 2, SM-20)

PHD1 (Egln2), PHD-2 (Egln1), and PHD3 (Egln3) are members of the Egln family of proline hydroxylases. EGLN family, which is known to be cellular oxygen sensor, initiates the first step of HIF degradation through the VHL/ubiquitin pathway. PHD1 and PHD2 can hydroxylate both proline 402 and proline 564, but PHD3 can only hydroxylate proline 564. In addition to HIF, PHD enzymes have also has been shown to catalyze the hydroxylation of RNA polymerase subunits and myogenin.\n\nApplications:\nSuitable for use in Immunofluorescence, ELISA, and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nImmunofluorescence: 1:500-1:1,000\nWestern Blot:1:500-1:1,000\nOptimal dilutions to be determined by the researcher.\n\nHybridoma:\nMouse F0 myeloma cells with spleen cells from BALB/c mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P3374-03

Size

100ul

Applications

ELISA, IF, WB

Hosts

Mouse

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

P Type

Mab

Purity

Purified by ammonium sulfate precipitation.

Isotype

IgG2b,k

References

1. Ozolins TR, et al., (2009) Biochem Biophys Res Commun 390(3):372-6 2. Haffey WD, et al., (2009) Adv Enzyme Regul 49(1):121-32 3. Dao JH, et al., (2009) Anal Biochem 384(2):213-23.

Additional Info

Recognizes human PHD2.

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