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PHF5A, Recombinant, Human, aa1-110, His-Tag (PHD Finger Protein 5A, bK223H9.2, INI, Rds3, SAP14b, SF3b14b)

Cat no: 137158

PHF5A, Recombinant, Human, aa1-110, His-Tag (PHD Finger Protein 5A, bK223H9.2, INI, Rds3, SAP14b, SF3b14b)

PHF5A is a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. PHF5A contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. \n\nSource: \nRecombinant protein corresponding to human PHF5A, fused to His-tag at N-terminal expressed in E.coli.\n\nMolecular Weight: \n~14.8kD (133aa) (MALDI-TOF)\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGSMAKHHPD LIFCRKQAGV AIGRLCEKCD GKCVICDSYV RPCTLVRICD ECNYGSYQGR CVICGGPGVS DAYYCKECTI QEKDRDGCPK IVNLGSSKTD LFYERKKYGF KKR\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

137158

Size

20ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 7.5, 0.15M NaCl, 10% glycerol, 1mM DTT.\n

Purity

~85% (SDS-PAGE)

References

1. Wagner SA, et al. (2011) Mol Cell Proteomics, PMID 21890473.

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Applications

ELISA

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Hum

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IF

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Mouse

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ELISA, WB

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Mouse

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Hum

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Applications

ELISA, FC, WB

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Mouse

Reactivities

Hum

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Applications

ELISA, FC, IHC, WB

Hosts

Mouse

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IHC, WB

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Rabbit

Reactivities

Hum

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Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

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