Phleomycin, Streptomyces verticillus

This unique antibiotic shows high toxicity in mammalian cells, plants, yeasts and bacteria. Ocin acts by binding to DNA and cleaving it causing cell death. Ocin is a copper-chelated glycopeptide antibiotic isolated from culture broth of a Streptomyces verticullus mutant and is part of the structurally related group of Bleomycin/Phleomycin type antibiotics. \n\nThe product of Sh ble, (Streptoalloteichus hindustanus bleomycin-resistance) gene confers resistance to Zeocin and Zeocell. The 14kD protein stoichiometrically binds to Zeocin and inhibits DNA strand cleavage and subsequent cell death.\n\nProduced by a Streptomyces verticullus mutant and is part of the structurally related group of Bleomycin/Phleomycin type antibiotics. Used as a selective agent for the incorporation of the Sh ble gene which encodes a 13,665 Dalton protein. The protein binds to the antibiotic and prevents binding to DNA. \n\nPhleomycin is a glycopeptide antibiotic of the bleomycin family, isolated from a mutant strain of Streptomyces verticullus. It binds and intercalates DNA thus destroying the integrity of the double helix. Use of phleomycin is recommended for cells poorly sensitive to Zeocin. Phleomycin is active against most bacteria, filamentous fungi, yeast, plant and animal cells.\n\nApplications:\nUsed as a selective agent for the incorporation of the Sh ble gene which encodes a 13.665kD protein. The protein binds to the antibiotic and prevents binding to DNA.\n \nBacilllus Subtilis 168:\n1.5-4.5ug/ml\n\nE.Coli:\nMC1061 sensitive strain: 1.5-4.5ug/ml\nMC1061 resistant strain: 1500-4500ug/ml\nTop 10 sensitive strain: 1.5-4.5ug/ml\nTop 10-pCDNA3Zeo (resistant strain): 1500-4500ug/ml\n\nPichia pastoris X33: \n1.5-4.5ug/ml\n\nSaccharomyces:\nOL1 sensitive strain: 0.45-1.5ug/ml\nOL1 resistant strain: >300ug/ml\n\nFilmaentous Fungi:\nTrichoderma reesie sensitive strain: <30ug/ml\n\nAnimal Cell Lines:\n293PS: 50-100ug/ml\nB16: 50ug/ml\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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