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Phosphatase, Alkaline (Alkaline Phosphatase, AP) (Biotin)

Cat no: P4071-38B

Phosphatase, Alkaline (Alkaline Phosphatase, AP) (Biotin)

The alkaline phosphatase used for this product is isolated from calf intestine. The very high specific activity of this enzyme is retained during biotinylation procedure. Biotinylated alkaline phosphatase used in conjunction with Avidin D provides one of the most sensitive enzyme detection systems available. For some solid-phase assay systems, the use of biotinylated alkaline phosphatase provides a more sensitive system than the Avidin D conjugate. This enzyme also offers a potential for use as a second marker in immunohistochemical staining.\n\nApplications: \nSuitable for use in ELISA. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nActivity:\n2200 units/mg protein. One unit = 1 umole of p-nitrophenol liberated from p-nitrophenylphosphate per minute t 37C (pH 9.5)\n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4071-38B

Size

1mg

Applications

ELISA

Conjugates

Biotin

Form

Supplied as a liquid in 10mM HEPES, 015M sodium chloride, 1mM mMg++, 1mM Zn++, pH 7.5, 0.08% azide. Labeled with Biotin.

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Applications

ELISA

Reactivities

Hum

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Applications

IF

Hosts

Mouse

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Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

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Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
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