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Phosphatase, Alkaline, BioAssay(TM) Kit

Cat no: P4071-07

Phosphatase, Alkaline, BioAssay(TM) Kit

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in alkaline buffer and produces an organic radical and inorganic phosphate. Changes in alkaline phosphatase level and activity are associated with various disease states in the liver and bone. The Alkaline Phosphatase Assay Kit is a highly sensitive, simple, direct and HTS-ready colorimetric assay designed to measure ALP activity in serum and biological samples. It contains 10 substrate tablets providing convenience for multiple usages. The kit uses pnitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (lambda-max= 405nm) when dephosphorylated by ALP. The Kit can detect 10-250uU ALP in samples.\n\nKit Components:\nALP *Assay Buffer 1x100ml\npNPP (10 TAB) 1x1vial\nALP Enzyme 1x1vial\nStop Solution 1x10ml.\n\nStorage and Stability:\nStore the kit at -20 degrees C, protect from light. Allow *Assay Buffer to warm to RT before use. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4071-07

Size

500Tests

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