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Phosphatidylcholine BioAssay(TM) Kit

Cat no: P4071-74T

Phosphatidylcholine BioAssay(TM) Kit

Phosphatidylcholine (PC) is a phospholipid which incorporates choline as the headgroup of the lipid. PC is a major constituent of biological membranes and is involved in cell signaling through release of choline by phospholipase D leaving the second messenger phosphatidic acid. This Phosphatidylcholine Assay Kit is a simple convenient means of measuring Phosphatidylcholine in a variety of biological samples. The kit utilizes an enzyme-coupled assay in which PC is hydrolyzed, releasing choline which is subsequently oxidized resulting in development of the OxiRed probe to generate fluorescence (Ex/Em 535nm / 587nm) and absorbance (570 nm). Phosphatidylcholine kit measures PC in the range of 0.1-10nmol per sample. PC is present in serum at ~ 0.2-2.5mM (~50-200mg/dL)\n\nKit Components:\n1. PC Assay Buffer, 1x25ml\n2. OxiRed Probe, 1x200ul\n3. PC Hydrolysis Enzyme, 1x1vial\n4. PC Development Mix, 1x1vial\n5. PC Standard (10umol), 1x1vial\n\nStorage and Stability:\nStore components at -20 degrees C, protect from light. Stable for 3 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4071-74T

Size

100Tests

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