Phosphodiesterase 5A (Phosphodiesterase 5A cGMP-specific, PDE5A, PDE-5A, PDE5A1, cGMP Binding cGMP Specific 3' 5' Cyclic Nucleotide Phosphodiesterase, cGMP-binding cGMP-specific 3

The cyclic monophosphate nucleotides are found ubiquitously in mammalian cells and act as second messenger transducers to effect the intracellular actions of a variety of G-protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cGMP in various tissues is phosphodiesterase type 5A. The cGMP is involved in nitric oxide signaling as well as cell signaling associated with natriuretic peptides and gulanylins. Some of the intracellular biding cites for the cGMP include cyclic nucleotide gated ion channels, cGMP-dependent protein kinases, and cyclic GMP biding phosphodiesterases (cGB-PDEs). The cGB-PDEs include PDE2, PDE3, PDE5, PDE5 and PDE10; the members of these families contain various structural and functional motifs that are conserved. Most of these proteins contain dimeric subunits that contain a highly conserved cGMP binding site and a phosphodiesterase catalytic site. \n\nApplications: \nSuitable for use in Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500\nImmunoprecipitation: 1:250\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nRat lung homogenate\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4072-05B

Size

100ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Rybalkin SD et al. J Biol Chem 277:3310-7 (2002). <PUBMED:11723116> 2.Murthy KS Biochem J 360:199-208 (2001). <PUBMED:11696008>McAllister-Lucas LM et al. The structure of a bovine lung cGMP-binding, cGMP-specific phosphodiesterase deduced from a cDNA clone. J Biol Chem 268:22863-73 (1993). <PUBMED:8226796>Farooqui SM et al. Antibodies against synthetic peptides predicted from the nucleotide sequence of D2 receptor recognize native dopamine receptor protein in rat striatum. J Neurochem 57:1363-9 (1991). <PUBMED:1832709>

Additional Info

Recognizes rat Phosphodiesterase 5A. Detects 99kD and 89kD PDE5A proteins in various tissues. Species Crossreactivity: human and mouse .