Phosphodiesterase 6 delta, Recombinant, Human (Phosphodiesterase 6D cGMP Specific Rod delta, PDE6 delta, PDE6D, PDED, GMP-PDE delta, Protein p17, Retinal Rod Rhodopsin Sensitive cGMP 3' 5' Cyclic Phosphodiesterase Subunit delta)

Human PDE6D was originally identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. Catalytically active PDE6 is a heterodimer (ab) that is regulated by two inhibitory g subunits. As PDE6D does not modify the catalytic properties of PDEab, its function is still unresolved. Recombinant human PDE6D, fused to His-tag at C-terminus, was expressed in E. coli.\n\nRecombinant protein corresponding to aa1-150 of human PDE6D with a 6x His tagged expressed in E. coli (NP_002592).\n\nAmino Acid Sequence:\nMSAKDERARE ILRGFKLNWM NLRDAETGKI LWQGTEDLSV PGVEHEARVP KKILKCKAVS RELNFSSTEQ MEKFRLEQKV YFKGQCLEEW FFEFGFVIPN STNTWQSLIE AAPESQMMPA SVLTGNVIIE TKFFDDDLLV STSRVRLFYV GSHHHHHH\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4072-06E

Size

100ug

Applications

WB

Form

Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 100mM sodium chloride, 10% glycerol.

Purity

~90% (SDS-PAGE). Purified by using conventional chromatography techniques.

References

1. Zhang H., et al, (2007) Proc Natl Acad Sci U S A. 104(21):8857-62. 2. Dekomien., G. et al. (2003) Genet Sel Evol. 35(4):445-56.

Alternative Names

Phosphodiesterase 6D cGMP Specific, Retinal Rod Photoreceptor cGMP Phosphodiesterase delta, Retinal Rod Rhodopsin-sensitive cGMP 3',5'-cyclic Phosphodiesterase Subunit delta