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Phosphoinositide 3 Kinase Enhancer, CT (PIKE, ANK Repeat and PH Domains 2, Arf GAP with GTP Binding Protein-like, Centaurin gamma 1, Centg1, Cnt2, GTP Binding and GTPase Activating Protein 2, Nuclear GTPase PIKE)

Cat no: P4073-38K

Phosphoinositide 3 Kinase Enhancer, CT (PIKE, ANK Repeat and PH Domains 2, Arf GAP with GTP Binding Protein-like, Centaurin gamma 1, Centg1, Cnt2, GTP Binding and GTPase Activating Protein 2, Nuclear GTPase PIKE)

PIKE-L is the longer of the two (124kDa) and participates in the prevention of neuronal apoptosis by enhancing PI3 kinase activity. It does so by binding to the adaptor protein Homer, linking to metabotropic glutamate receptors. The shorter PIKE-A isoform is also known as Centaurin gamma 1. It does not stimulate PI3 kinase but may protect cells from apoptosis by stimulating Akt. Overexpression of PIKE-A enhances Akt activity and promotes cancer cell invasion. PIKE-A also regulates the adaptor protein 1-dependent trafficking of proteins in the endosomal system. PIKE-L is found in the cytoplasm and nucleus, and is brain-specific, whereas PIKE-A is ubiquitously expressed and found in the cytoplasm only.\n\nApplications:\nSuitable for use in Western Blot, Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.5-2ug/ml\nImmunohistochemistry (Paraffin): 10ug/ml\nOptimal dilutions to be determined by the researcher.\n\nImmunohistochemistry Positive Control Tissue:\nMouse Brain\n\nRecommended Secondary Antibodies:\nGoat Anti Rabbit IgG (H&L) HRP\nSheep Anti Rabbit IgG Biotin\nGoat Anti Rabbit IgG (Fc) FITC, HRP\nGoat Anti Rabbit IgG (H&L) HRP\nSheep Anti Rabbit IgG FITC\nSheep Anti Rabbit IgG RPE\nSheep Anti Rabbit IgG DyLight(TM)488, DyLight(TM)549, DyLight(TM)649\nSheep Anti Rabbit IgG HRP\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4073-38K

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Form

Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.

P Type

Pab

Purity

Purified by Ion Exchange chromatography.

Isotype

IgG

Additional Info

Recognizes the C-terminus of phosphoinositide 3-kinase enhancer (PIKE). Recognizes both PIKE-L and PIKE-A. Species crossreactivity: Mouse.

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