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Phosphoinositide 3 Kinase, p110 alpha (PI3K)

Cat no: P4073-26G1

Phosphoinositide 3 Kinase, p110 alpha (PI3K)

PI3 Kinase is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85kD regulatory subunit and a 110kD catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors. PI3 Kinase is also activated by Ras, and the beta:gamma subunits of heterodimeric G-Proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.\n\nApplications: \nSuitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution: \nImmunofluorescence: 1:100-1:250\nWestern Blot: 1:5000\nImmunoprecipitation: 1:30\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4073-26G1

Size

100ul

Applications

IF, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA and 40% glycerol.

P Type

Mab

Purity

Purified immunoglobulin

Isotype

IgG

References

1. Knight, Z.A., et al., Cell 125: 733-747 (2006). 2. Carpenter, C.L., et al., J. Biol. Chem. 265: 19,704-19,711 (1990).

Additional Info

Recognizes the C-terminal domain of human PI3 Kinase, p110a.

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