Phosphoinositide 3 Kinase, p85 alpha (PI3K)\n

PI3-Kinases (PI3-Ks) are a family of lipid kinases that are implicated in signal transduction. PI3-K consists of two subunits; the regulatory subunit p85 and the catalytic subunit p110 (1-2). In quiescent cells p85 maintains p110 in a low activity state. Upon growth factor stimulation, p85 directly interacts with phosphotyrosine residues of activated growth factor receptors or adaptor proteins leading to activation of p110. p85 exists in two isoforms: p85 , including two short forms termed p50 and p55/AS53 and p85 including short form p55 PIK/p55 (3). The p85 adaptor protein uses the inter-SH2 (iSH2) region to bind p110 and employs SH3 and SH2 domains, phosphotyrosine residues, and proline-rich motifs to interact with activated tyrosine kinases such as BCR/ABL and NPM/ALK or adaptor proteins including Shc, c-Cbl, Crk-L, and Gab2 (4-5).\n\nApplications: \nSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:60\nWestern Blot: 1:1,000-2,000\nImmunohistochemistry: 1:100-250\nImmunocytochemistry: 1:250-500\nImmunoprecipitation: 1:50\n\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Freeze at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

P4073-30C

Size

100ul

Applications

FC, ICC, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Rat

Form

Supplied as a liquid.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Otsu et al. (1992) Cell 65, 91-104 2. Hiles et al. (1991) Cell 70, 419-429 3. Inukai et al. (1997) J. Biol. Chem. 272, 7873-7882 4. Klippel, A., J. A. Escobedo, Q. Hu, and L. T. Williams. Mol. Cell. Biol. 13:5560-5566. 5. Jain, S. K., W. Y. Langdon, and L. Varticovski. 1997.Oncogene 14:2217-2228

Additional Info

Recognizes human PI3-K 85 alpha