Phosphoinositide 3 Kinase, Regulatory Subunit1, p85 alpha (PI3K, GRB1, p85-ALPHA, PtdIns-3-Kinase p85-alpha)

Applications: \nSuitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 1:50\nWestern Blot: 1:2000 A 1:2000 Detected endogenous PI3K, p85a in lysates from A431, MCF-7, and Raji cells. Lysates from A431, MCF-7 and Raji cells were resolved by electrophoresis, transferred to PVDF and probed with P4073-26J (1:2000). Proteins were visualized using goat anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection.\nImmunohistochemistry: 1:200 Used paraffin-embedded human breast carcinoma tissue\nImmunocytochemistry (IF): 1:250-1:500. Used paraformaldehyde fixed HeLa cells\nImmunoprecipitation: 1:50. Immuprecipitates PI3K, p85a using Protein G agarose beads.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies\nI1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb\nI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb\nI1904-46Q: IgG, H&L (HRP) Pab Gt xRb\nI1904-40D: IgG, H&L (FITC) Pab Gt xRb\nI1904-41: IgG, X-Adsorbed (FITC) Pab Gt xRb \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4073-26J

Size

100ul

Applications

FC, ICC, IF, IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.

P Type

Mab

Purity

Serum

Isotype

IgG

References

1. Kwon, Mijin, et al (2006). Recruitment of the tyrosine phosphatase Src homology 2 domain tyrosine phosphatase-2 to the p85 subunit of phosphatidylinositol-3 (PI-3) kinase is required for insulin-like growth factor-I-dependent PI-3 kinase activation in smooth muscle cells. Endocrinology 147: 1458-65. 2. Lee, Ho-Young, et al (2003). Evidence that phosphatidylinositol 3-kinase- and mitogen-activated protein kinase kinase-4/c- Jun NH2-terminal kinase-dependent Pathways cooperate to maintain lung cancer cell survival. J Biol Chem 278: 23630-8.

Additional Info

Recognizes the C-terminal domain of PI3 Kinase, p85a subunit at 85kD. Species Crossreactivity: human, mouse and rat.