Phospholipase A2, Secretory, Group IB (PLA2G1B, phospholipase A2, MGC119834, MGC119835, Phosphatidylcholine 2-acylhydrolase, Phospholipase A2 precursor, PLA2, PLA2A, PPLA2)

Group IB secretory phospholipase A2 (sPLA2-IB) mediates cell proliferation, cell migration, hormone release and eicosanoid production via its receptor in peripheral tissues. In the CNS, high-affinity binding sites of sPLA2-IB have been documented. sPLA2-IB induced neuronal cell death in a concentration dependent manner depending on PGD2 metabolites, especially Delta12-PGJ2 that might mediate sPLA2-IB-induced apoptosis. The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of lowmolecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nPeptide ELISA Titer: 1:64,000\nWestern Blot: 0.01ug/ml; ~17-18kD band observed in Human Pancrease lysates (calcuated MW: 16.4kD according to NP_000919.1). In transfected HEK293 transiently expressing PLAG1B a band of ~18kD is observed. This band is not observed in the non-transfected HEK293.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nHuman Pancreas lysates\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4074-06D

Size

100ug

Applications

ELISA, WB

Hosts

Goat

Reactivities

Hum

Form

Supplied as a liquid in Tris, pH 7.3, 0.5% BSA, 0.02% sodium azide.

P Type

Pab

Purity

Purified by peptide affinity chromatography.

Isotype

IgG

References

1. Kolko M, Christoffersen NR, Varoqui H, Bazan NG. Expression and induction of secretory phospholipase A2 group IB in brain. Mol Neurobiol. 2005 Nov;25(7):1107-22.

Additional Info

Recognizes human Phospholipase A2, Secretory, Group IB. \nSpecies sequence homology: canine.