Phospholipase C, gamma 1, phosphorylated (Tyr783) (PLCg1)

Enzymes of the phospholipase C family catalyze the hydrolysis of phospholipids to yield diacylglycerols and water-soluble phosphorylated derivatives of the lipid head groups (1). Phospholipase C gamma 1 (PLC gamma 1) hydrolyses phosphatidylinositol-4,5-bisphosphate to the second messengers inositol-1,4,5-trisphosphate and diacylglycerol. PLC-gamma 1 also has mitogenic activity upon growth-factor-dependent tyrosine phosphorylation; however, this activity is not dependent on the phospholipase activity of PLC gamma 1, but requires an SH3 domain (2). Also, results indicate a lipase-independent role of PLC gamma in the physiological agonist-induced activation of Ca2+ entry (3). Insulin increased PLCgamma1 tyrosine phosphorylation at Tyr-783 and its colocalization with the IR in punctated structures enriched in cortical actin at the dorsal plasma membrane. This association was found to be independent of PLC gamma1 Src homology 2 domains (4).\n\nApplications: \nSuitable for use in Immunocytochemistry, Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:5000 \nImmunocytochemistry: 1:100-1:250\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

P4074-42V

Size

100ul

Applications

ICC, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Argeson AC, et al. Genomics 25(1):29-35, 1995 2. Ye, K, et al. Nature 415(6871):514-4, 2002\n3. Patterson RL, et al Cell, 111(4):529-41, 2002 4. Kwon YK et al. J Cell Biol 163(2):375-84, 2003 \n \n\n

Additional Info

Recognizes human PLC gamma 1 phosphorylated on tyrosine 783. \n