Phospholipase D1 (PLD1) (NT)

Phospholipase D (PLD) cleaves phosphatidylcholine in response to a variety of cell stimuli to release phosphatidic acid, which is associated with a number of cellular responses including regulated secretion, mitogenesis, and cytoskeletal changes. Human PLD1 does not appear to be an abundant message in any tissue tested whereas levels of human PLD2 mRNA apparently were higher and more variable (1). PLD) has been implicated in a variety of cellular processes including vesicular transport, the respiratory burst, and mitogenesis. PLD1, first cloned from human, is activated by small GTPases such as ADP-ribosylation factor (ARF) and RhoA (2). Phosphoinositides are localized in various intracellular compartments and can regulate a number of intracellular functions, such as cytoskeletal dynamics and membrane trafficking. PLDs are regulated enzymes that hydrolyse phosphatidylcholine (PtdCho) to generate the putative second messenger phosphatidic acid (PtdOH). In vitro, PLDs have an absolute requirement for higher phosphorylated inositides, such as phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P(2)] (3).\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot, Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500\nImmunocytochemistry: 1:100-1:250\nFlow Cytometry: 1:40\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

P4074-50E

Size

100ul

Applications

FC, ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Steed PM, et al. FASEB J 12(13):1309-17, 1998 2. Lopez I, et al. J Biol Chem 273(21):12846-52, 1998 3. Divecha N, et al. EMBO J 19(20):5440-9, 2000\n

Additional Info

Recognizes human PLD1, NT. Species crossreactivity: mouse, rat