Phosphoserine

Changes in the serine/threonine phosphorylation state of a protein in response to various external stimuli can have profound effects on cellular signal transduction, apoptosis and carcinogenesis. The reagents, including phosphorylated proteins, peptides, and antibodies against the phosphospecific amino acid, are important tools to explore the activation of serine, threonine or tyrosine containing proteins. An aberrant protein phosphorylation is a hallmark of human disease, and the enzymes, particularly protein kinases, which control protein phosphorylation are recognized as a major new drug target family.\n\nApplications: \nSuitable for use in Western Blot, Dot Blot and Indirect ELISA. Other applications have not been tested.\n\nRecommended Dilutions:\nWestern Blot (Indirect): 1:500-1:1000\nIndirect ELISA: 1:8000-16,000\nNote: Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead.\nOptimal dilutions to be determined by researcher.\n\nPositive Control: \nRat brain cortex and extract at ~50kD.\n\nHybridoma:\nMouse myeloma cells with splenocytes from immunized mice.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4076-01

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Rat

Form

Supplied as a liquid, 15mM sodium azide.

P Type

Mab

Purity

Ascites

Isotype

IgG1

References

1. Abu-Lawi, K.I. & Sultzer, B.M., Infect. Immunol. 63: 498-502 (1995). 2. Hunter, T. and Cooper, J.A., Annu. Rev. Biochem. 54: 897 (1985). 3. Heffetz, D., et al., Methods Enzymol. 201: 44 (1991). 4. Alexander, D. and Cantrell, D., Immunol. Today 10: 200 (1989). 5. Levine, L., et al., J. Immunol. Methods 124: 239 (1989).

Additional Info

Recognizes phosphorylated serine both as free amino acid or when coupled to carriers such as BSA or KLH. Does not crossreact with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody because of steric hindrance of the recognition site. Species Crossreactivity: rat.