Phosphothreonine

Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested. \n\nRecommended Dilutions: \nELISA: 1:1000 to 1:5000, assayed against phosphothreonine conjugated BSA using IgG, H&L (HRP) Pab Gt xMo for detection.\nWestern Blot: 1:500 \nOptimal dilutions to be determined by the researcher.\n\nRecommended secondary antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\nI1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo\n\nHybridoma: \nSp2/0 myeloma cells with spleen cells from Balb/c mice. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4077-11

Size

100ul

Applications

ELISA, WB

Hosts

Mouse

Form

Supplied as a liquid, 0.01% sodium azide before the addition of glycerol to 40%. No buffer or stabilizers added. No stabilizing proteins added.

P Type

Mab

Purity

Ascites

Isotype

IgG1,k

References

1. Frackelton, AR, et al. (1983) Mol. Cell. Biol. 3:1343. 2. Edleman, AM, et al. (1987) Annu. Rev. Biochem. 56:567. 3. Hunter, T. (1987) Cell 50:823. 4. Sengupta, A, et al, (1988)

Additional Info

Reacts specifically with phosphothreonine. Shows minimal reactivity by ELISA and competitive ELISA with phosphoserine or phosphotyrosine. Reacts with free phospho-amino acid, phosphothreonine conjugated to carriers such as thyroglobulin or BSA, and detects the presence of phosphothreonine in proteins of both unstimulated and stimulated cell Reactivity is specific for phosphothreonine and minimal crossreactivity is observed against phosphoserine or phosphotyrosine lysates.