Phosphotyrosine

Phosphorylation and dephosphorylation of cellular proteins are central steps in transducing extracellular signals to the cell nucleus. Phosphorylated epitopes may serve as docking sites for the assembly of protein complexes or may alter the 3-dimensional protein structure thus modulating enzymatic activity or the ability to undergo protein-protein interactions. Modification of proteins on tyrosine residues is mediated by protein tyrosine kinases. Tyrosine phosphorylation may alter the biological activity or mediate the assembly of protein complexes via interaction of phosphotyrosine residues with SH2 or PID domains.\n\nApplications: \nSuitable for use in ELISA, Immunoprecipitation, and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 0.05ug/ml\nWestern Blot: 0.5ug/ml \nImmunoprecipitation: 1-10ug per 10e6 vanadate treated A431 cells \nOptimal dilutions to be determined by the researcher.\n\nPositive Control (supplied): \nPhosphotyrosine Molecular Weight Marker. Supplied as a lyophilized powder. Reconstitute with 200ul sterile, ddH2O.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4078-05B

Size

100ug

Applications

ELISA, IP, WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Can

Form

Supplied as a lyophilized powder from 2X PBS, PEG, sucrose, 0.09% sodium azide. Reconstitute in 1ml distilled water.

P Type

Mab

Purity

Purified by ultrafiltration and size exclusion chromatography.

Isotype

IgG1

Additional Info

Recognizes phosphotyrosine in the context of the surrounding amino acids, tolerating charged amino acids directly neighbored to phosphotyrosine. Epitope: