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PI 3-Kinase p85 alpha (Phosphatidylinositol-PI-3-Kinase)

Cat no: P4185-34L

PI 3-Kinase p85 alpha (Phosphatidylinositol-PI-3-Kinase)

PI 3-Kinases phosphorylate the inositol ring on a variety of membrane-associated phosphatidylinositols. Class I phosphoinositide 3-kinases (PI 3-Kinases) are heterodimeric proteins that consist of a catalytic subunit of 110-120kD and an associated regulatory subunit. p85a is one of the regulatory subunits that associate with the class IA PI 3-Kinases. Composed of one SH3 and two SH2 domains, p85a functions as an adaptor, coupling catalytic p110 to activated receptor tyrosine kinases. \n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1ug/ml\nOptimal dilutions to be determined by the researcher.\n\nHybridoma: \nMouse myeloma cells with B cells obtained from a mouse immunized with purified, E. coli-derived, recombinant human PI 3-Kinase p85a (aa519-644).\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4185-34L

Size

100ug

Applications

WB

Hosts

Mouse

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG2B

Additional Info

Recognizes human PI 3-Kinase p85 alpha. Species Crossreactivity: mouse, rat.

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SUPPLIER INFO

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info

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