logo
logo
PI 3-Kinase p85 (Phosphatidylinositol-PI-3-Kinase) (Agarose)

Cat no: P4185-27

PI 3-Kinase p85 (Phosphatidylinositol-PI-3-Kinase) (Agarose)

Applications: \nSuitable for use in Immunoprecipitation and Affinity Purification. Other applications not tested.\n\nRecommended Dilution:\nImmunoprecipitation:10ug (20ul of gel slurry) immunoprecipitates PI 3- Kinase from 500ug of a RIPA lysate of EGF-stimulated human A431 carcinoma cells, as shown by immunoblot analysis of the immunoprecipitate. Note that boiling the beads in SDS-PAGE sample buffer releases a small amount of the IgG heavy chain (~ 54kD) and the non-covalently bound IgG light chain (~28kD). \n\nAffinity Purification: The antibody-agarose may be useful for affinity purification of proteins that bind to the specific antigen. This lot of antibody-agarose can be reused a minimum of three times after elution of human PI 3-Kinase by either high pH or high salt. PI 3-Kinase may be released from the antibody-agarose by the agents listed below:\n\nElution Profile: \n\nElution Buffer Relative % Antigen Released \nSDS-PAGE sample buffer 100\n 0.1M Glycine, pH 2.5 50 \n0.1M TEA, pH 11.5 70 \n3.5M MgCl2, pH 7.2 20 \n\nNote: It is recommended to wash the agarose beads with appropriate buffer prior to use to remove sodium azide.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

P4185-27

Size

200ug

Applications

IP, WB

Hosts

Rabbit

Reactivities

Rat

Conjugates

Agarose

Form

Provided as a 50% slurry. Beads are suspended in PBS, pH 7.4, 0.05% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes rat PI 3-Kinase p85 (Phosphatidylinositol-PI-3-Kinase) (Agarose).

Read more on Supplier website

Applications

ELISA

Reactivities

Hum

More info

Applications

IF

Hosts

Mouse

More info

Applications

ELISA, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, WB

Hosts

Mouse

Reactivities

Hum

More info

Applications

ELISA, FC, IHC, WB

Hosts

Mouse

More info

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

More info
Advertisehere
MtoZ Horizontal banner
MtoZ Biolabs

Latest promotion FROM:

MtoZ Biolabs
View promotion

Latest promotions

Spend less time on DNA cleanup so you can do more science. The MSB Spin PCRapace is the fastest way to purify your DNA from PCR, restriction digestion, and...

New brilliant antibodies, and new lower prices!For flow cytometry reagents in general, \"bright is better.\" The violet-excitable BD Horizon™ BV421 and...

As an incentive to qualify our BSA, we are offering a 20% discount when you purchase your first 100g, 500g or 1000g of any grade of Bovine Serum Albumin....

It is not every day that you are given something for nothing. We are giving away additional spectrophotometer software.Cecil Instruments have enhanced the...

We're so sure that you'll prefer Cayman Assay kits over your present brand that we're willing to give you a free assay kit to prove it!

10% Discount on 2 Rabbit Polyclonal Antibody Service. With over 20 years experience, SDIX has developed into the premier US custom antibody producer,...

For the past decade scientists have extensively used ATS secondary toxin conjugates to make their own targeted toxins for in vitro use.The ability to combine...

Did your supplier increase the price of Fetal Bovine Serum? Did they substitute the US Origin with USDA? Well say no more! Innovative Research is still...

Bulk Cytokines with Custom Vialing.20 - 50% off cytokines, growth factors, chemokines and more...For a limited time Cell Sciences is offering substantial...

Jenway’s 73 series spectrophotometer range provides four models with a narrow spectral bandwidth of 5nm and an absorbance range of –0.3 to 2.5A,...

Are you planning to have a customised antibody made for your research?Since 2000, Everest has been producing a catalog containing thousands of affinity...