PI3K p85(beta) (R458) pAb

Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85(alpha) and p85(beta)), each possessing one SH3 and two SH2 domains. Various p110 isoforms have been identified. p110(alpha) and p110(beta) interact with p85(alpha), and p110(alpha) has also been shown to interact with p85(beta) in vitro. p110(delta) expression is restricted to white blood cells. It has been shown to bind p85(alpha) and p85(beta), but it apparently does not phosphorylate these subunits. p110(delta) seems to have the capacity to autophosphorylate. p110(gamma) does not interact with the p85 subunits. It has been shown to be activated by (alpha) and (beta)(gamma) heterotrimeric G proteins.

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SPECIFICATIONS

Catalog Number

BS3184

Size

100ug/100ul

Applications

WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

liquid

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Antigen

Synthetic peptide, corresponding to amino acids 431-480 of Human PI3K p85(beta).

Species

PI 3-kinase p85(beta) (R458) pAb detects endogenous levels of PI 3-kinase p85(beta) protein.

Dilutions

WB: 1:500~1:1000

Swiss Prot

O00459

Storage Temp

Store at 4 degrees C short term. Aliquot and store at -20 degrees C long term. Avoid freeze-thaw cycles.

Concentration

1ug/ul

Storage Buffer

1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.

Additional Info

For research use only, not for use in diagnostic procedure.

Molecular Weight

~ 85 kDa

Alternative Names

p85; p85 beta; P85B; P85B; Phosphatidylinositol 3 kinase; Phosphatidylinositol 3 kinase regulatory beta subunit; Phosphatidylinositol 3 kinase regulatory subunit beta; Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2; Phosphatidylinositol 3 kinase; regulatory subunit; polypeptide 2 (p85 beta); Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; Phosphatidylinositol 3-kinase regulatory subunit beta; Phosphoinositide 3 kinase regulatory subunit 2 (beta); P