Pin 1, Recombinant, Human (Peptidyl-prolyl cis/trans Isomerase NIMA-interacting 1)

Synonyms: NIMA-interacting Protein 1\n\nHuman Pin 1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and\nessential for cell cycle regulation Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53, and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1. \n\nSequence: \nMADEEKLPPG/ WEKRMSRSSG/ RVYYFNHITN/ ASQWERPSGN/ SSSGGKNGQG/ EPARVRCSHL/ LVKHSQSRRP/ SSWRQEKITR/ TKEEALELIN/ GYIQKIKSGE/ EDFESLASQF/ SDCSSAKARG/ DLGAFSRGQM/ QKPFEDASFA/ LRTGEMSGPV/ FTDSGIHIIL/ RTE\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4186-95

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 0.1M sodium chloride, 5mM DTT, 20% glycerol.

Purity

(same/more than) 95% by SDS PAGE

References

1. Wulf, G.M., et al., J. Biol. Chem. 277(50): 47,976-47,979 (2002). 2. Hamdane, M., et al., J. Mol. Neurosci. 19(3): 275-287 (2002). 3. Zheng, H., et al., Nature 419(6909): 853-857 (2002). 4. Lu, K.P., et al., Nature 380(6574): 544-547 (1996). 5. Campbell, H.D., et al., Genomics 44(2): 157-162 (1997).