Pix, beta (Pix, COOL, p85SPR)

Beta-Pix is a p21-activated protein kinase (Pak)-interacting exchange factor that has been identified as a putative guanine nucleotide exchange factor (GEF) for Rac/Cdc42. Beta-Pix is expressed ubiquitously in all the tissue examined and other isoforms are expressed mainly in the central nervous system.\n\nApplications: \nSuitable for use in ELISA, Immunocytochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA (Direct and Indirect): 1:5000-1:10,000\nImmunocytochemistry: 1:1000\nWestern Blot: 1:5000-1:10,000 using ECL Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Incubate for 1 hour at RT.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P4210-59A

Size

100ug

Applications

ELISA, ICC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4. No preservative added.

P Type

Pab

Purity

Purified

Isotype

IgG

References

Product Specific Literature References 1. Cloning of a SH3 domain-containing proline-rich protein, p85SPR, and its localization in focal adhesion: W.K. Oh, et al.; BBRC 235, 794 (1997) 2. betaPix-b(L), a novel isoform of betaPix, is generated by alternative translation: S. Rhee, et al.; BBRC 318, 415 (2004) GENERAL LITERATURE REFERENCES 1. A novel regulator of p21-activated kinases: S. Bagrodia, et al.; J. Biol. Chem. 273, 23633 (1998) 2. Leucine zipper-mediated homodimerization of the p21-activated kinase-interacting factor, beta Pix. Implication for a role in cytoskeletal reorganization: S. Kim, et al.; J. Biol. Chem. 276, 10581 (2001) 3. Human immunodeficiency virus type 1 Nef selectively associates with a catalytically active subpopulation of p21-activated kinase 2 (PAK2) independently of PAK2 binding to Nck or beta-PIX: G.H. Renkema, et al.; J. Virol. 75, 2154 (2001) 4. Genomic structure of the human beta-PIX gene and its alteration in gastric cancer: Z. Li, et al.; Cancer Lett. 177, 203 (2002) 5. Phosphorylation of p85 beta PIX, a Rac/Cdc42-specific guanine nucleotide exchange factor, via the Ras/ERK/PAK2 pathway is required for basic fibroblast growth factor-induced neurite outgrowth: E.Y. Shin, et al.; J. Biol. Chem. 277, 44417 (2002) 6. Mammalian Scribble forms a tight complex with the betaPIX exchange factor: S. Audebert, et al.; Curr. Biol. 14, 987 (2004)

Additional Info

Recognizes the multiple splice variants of Beta-Pix-a, -b, bL, -c and -d isoforms. Species Crossreactivity: Human, mouse and rat