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PKCd, phosphorylated (Ser645) (Protein Kinase C delta Type, nPKC-delta, PRKCD)

Cat no: P9103-19U

PKCd, phosphorylated (Ser645) (Protein Kinase C delta Type, nPKC-delta, PRKCD)

PKCd a member of the novel PKC subfamily, is actively involved in cell apoptosis in a stimulus and tissue specific manner; it both regulates the expression and function of apoptotic related proteins and is itself a target for caspases. Activation of PKCd by various apoptotic stimuli results in the translocation of PKCd to distinct cellular compartments such as mitochondria, golgi and nucleus, and the differential translocation contributes to its different effects. In addition, phosphorylation of PKCd on distinct tyrosine residues and its association with specific apoptotic related proteins such as c-Abl, DNA-PK, p73 and lamin B are pivotal to its function in cell apoptosis. Human PKCd is mapped on chromosomal region 3p21.31.\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500-1:1000\nImmunohistochemistry (paraffin): 1:50-1:100\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nMCF7 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

P9103-19U

Size

50ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Brodie, C. et al. Apoptosis. 8:19-27 (2003).

Additional Info

Recognizes human PKCd, phosphorylated at Ser645. Species Crossreactivity: mouse and rat.

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