PLA2G4A, Recombinant, Human (Cytosolic Phospholipase A2, cPLA2, Phospholipase A2 Group IVA, Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Lysophospholipase, PLA2G4, CPLA2)

Cytosolic Phospholipase A2a (PLA2G4A) belongs to the group IV phospholipase A2 family (1). These enzymes hydrolyze the ester bond at the second position (sn-2) of membrane glycerophospholipids. The group IV phospholipase A2 (PLA2G4) family is comprised of six intracellular enzymes (2). PLA2G4A has a preference for arachidonic acid in the sn-2 position of phospholipids indicating its involvement in the metabolism of eicosanoids. Its active site is characterized by a serine and aspartic acid dyad. The overall structural feature displays two domains, a lipid binding C2 domain and a catalytic a/b hydrolase domain. PLA2G4A translocation to the membrane and activation is facilitated by a calcium-ion dependent conformational change in its C2 domain. In addition, phosphorylation of serine residues in PLA2G4A by protein kinases may be another regulatory mechanism for promoting the release of arachidonic acid. The knockout mouse studies of this enzyme indicate that the enzyme may play a major role in inflammatory diseases. Genetic ablation or reduction of PLA2G4A protected human amyloid precursor protein (APP) related pathogenesis from a mouse model (3). Mouse model studies of brain and lung cancer indicates that PLA2G4A may play a key regulatory role in angiogenesis of these tumors (4).\n\nRecombinant corresponding to aa1-749 from Human PLA2G4A fused with 6-His-tag at C-terminal expressed in Sf21 cells (NP_077734).\n\nMolecular Weight: \n~86kD\n\nBiological Activity:\nMeasured by its ability to hydrolyze 1-Hexadecanoyl-2-(1-pyrene-decanoyl)-sn-glycero-3-phosphocholine. The specific activity is >30pmol/min/ug.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4074-03R

Size

10ug

Form

Supplied as a liquid in 25mM Tris, 400mM sodium chloride, 10mM DTT, 0.02% Brij-35, pH 7.5

Purity

by SDS-PAGE under reducing conditions and visualized by colloidial Coomassie Blue stain at 5ug per lane. Endotoxin: (same/less than)1EU/1ug (LAL)

References

1. Burke, J. E. and E.A. Dennis (2009) J. Lipid Res. 50:S237. 2. Ghosh, M. et al. (2006) Prog. Lipid Res. 45:487. 3. Sanchez-Mejia, R.O. et al. (2008) Nat. Neuroscience 11:1311. 4. Linkous, A.G. et al. (2010) J. Natl. Cancer Inst. 102:1398.