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Plasmin Inhibitor, alpha, Human (a2 PI)

Cat no: P4256-10

Plasmin Inhibitor, alpha, Human (a2 PI)

Alpha 2 Plasmin Inhibitor and Fibrinolysis: The primary physiological inhibitor of plasmin is Alpha 2 Plasmin Inhibitor. The binding of Alpha 2 Plasmin Inhibitor to plasmin (ogen) plays a critical role in fibrinolysis. The binding of circulating free plasmin (ogen) by Alpha 2 Plasmin Inhibitor prevents both zymogen and enzyme from binding to fibrin and ultimately initiating fibrinolysis. Alpha 2 Plasmin Inhibitor already crosslinked to fibrin prevents plasmin catalytic action on local sites of fibrin deposition as well as impeding subsequent activation of plasminogen via plasminogen activator.\n

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SPECIFICATIONS

Catalog Number

P4256-10

Size

100ug

Form

Supplied as a liquid in 50mM potassium phosphate, 7.5mM KCl, 0.075mM EDTA, pH 7.4.

Purity

(same/more than)95% by SDS-PAGE. Alpha 2 Plasmin Inhibitor is prepared from fresh frozen plasma by a combination of ion exchange, affinity, and gel filtration chromatography steps. Our purification selects exclusively for the native plasmin-ogen-binding form. Purity is assessed by SDS-PAGE analysis and plasmin inhibition assay.

References

1 Aoki, N. et al., Methods Enzymol., 223, 185-197 (1993). 2. Moroi, M. and Aoki, N., J. Biol. Chem., 251, 5956 (1976). 3. Wiman, B. and Collen, D., J. Biol. Chem., 254, 9291 (1979). 4. Moroi, M. and Aoki, N., Thromb. Res., 10, 851 (1977). 5. Sakata, Y. and Aoki, N., J. Clin. Invest., 69, 536 (1982). 6. Aoki, N. et al., J. Clin. Invest., 63, 877 (1979). 7. Israels, E. D. et al., J. Clin. Invest., 52, 2398 (1973). 8. Holmes, W. E. et al., J. Biol. Chem., 262, 1659 (1987). 9. Kluft, C. and Los, N., Thromb. Res., 21, 65 (1981). 10. Tamaki, T. and Aoki, N., J. Biol. Chem., 257, 14767 (1982).

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