PLTP, Recombinant, Mouse (Phospholipid Transfer Protein)

Mouse PLTP Phospholipid transfer protein (PLTP; also lipid transfer protein II) is an 80kD, secreted glycoprotein, that belongs to the BPI/LBP/Plunc superfamily, BPI/LBP family of molecules (1). It is synthesized as a precursor with a 17aa signal sequence and a 476aa mature chain (SwissProt # P55065). The mature chain contains two BPI/LBP/CETP domains (aa21-239 and 263-463) and seven potential sites for N-linked glycosylation. Mature mouse PLTP shares 83% aa sequence identity with mature human PLTP (3). PLTP is expressed by a wide variety of tissues and is secreted by macrophages. Northern blot analysis shows the order of PLTP mRNA expression as follows: lung > adipose tissue, placenta, testis > brain > muscle, heart, liver (2). PLTP is a multifunctional, extracellular lipid transfer protein that plays a major role in lipoprotein metabolism and atherosclerosis (4-6). In addition to phospholipids, PLTP transfers several amphipatic compounds, including unesterified cholesterol, lipopolysaccharides, diacylglycerides, and alpha-tocopherol, the main isomer of vitamin E (4). According to several studies, PLTP not only affects plasma lipid and lipoprotein profiles, but also modulates cell physiology (4, 7-11). In mice, systemic PLTP deficiency proves to be atheroprotective, whereas PLTP overexpression is proatherogenic (4, 6). However, macrophage PLTP is shown to be atheroprotective (4, 6). \n\nSource: \nA DNA sequence encoding the mature mouse PLTP (Glu 18-Ala 493; AAH03782) \nwas fused with a 6X-His tag at the C-terminus and expressed in a mouse myeloma cell line, NS0. \n\nMolecular Mass: \nBased on N-terminal amino acid sequencing, the recombinant mouse PLTP starts at Glu 18 and has a calculated molecular mass of approximately 53.5kD. The recombinant mouse PLTP migrates as an approximately 70-85kD protein in SDS-PAGE under reducing conditions. \n\nActivity: \nMeasured by its ability to inhibit LPS-induced TNF-alpha secretion by Raw264.7 cells.\n\nStorage and Stability:\n12 months from date of receipt, 20 to 70 degrees C as supplied.\n1 month, 2 to 8 degrees C under sterile conditions after reconstitution.\n3 months, 20 to 70 degrees C under sterile conditions after reconstitution.\nUse a manual defrost freezer and avoid repeated freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

P4278-01

Size

25ug

Form

Supplied as a lyophilized powder in 0.2um sterile-filtered solution in PBS, 50ug of BSA per ug of protein. Reconstitute with 40-50% glycerol, PBS.

Purity

Purified (same/more than)95% (SDS-PAGE). Endotoxin: (same/less than)1.0 EU/1ug (LAL).

References

1. Jauhiainen, M. and C. Ehnholm, 2005, Methods 36:97. 7. Jiang, X.-C. et al., 1998, J. Biol. Chem. 273:15714. 2. Jiang, X.-C. and C. Bruce, 1995, J. Biol. Chem. 270:17133. 8. Jiang, X.-C. et al., 2005, J. Biol. Chem. 280:18336. 3. Albers, J.J. et al., 1995, Biochim. Biophys. Acta. 1258:27. 9. Desrumaux, C. et al., 2005, FASEB J. 19:296. 4. Ogier, N. et al., 2007, Arterioscler. Thromb. Vasc. Biol. 27:2407. 10. Drouineaud, V. et al., 2006, FASEB J. 20:794. 5. Huuskonen, J. and C. Ehnholm, 2000, Curr. Opin. Lipidol. 11:285. 11. Klein, A. et al., 2006, Arterioscler. Thromb. Vasc. Biol. 26:2160. 6. Valenta, D.T. et al., 2008, J. Lipid Res. 49:24.

Additional Info

Recognizes mouse PLTP.