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PlurES-1 (Pluripotent Human Embryonic Stem Cells-1)

Cat no: P4284

PlurES-1 (Pluripotent Human Embryonic Stem Cells-1)

Human embryonic stem cells (hESCs) have been traditionally characterized using a panel of antibodies to cell surface markers, such as SSEA-3, TRA-1-60, and TRA-1-81; however, none of these traditional markers are entirely specific to hES cells (Laslett et al., 2003). Therefore, there is a continuous quest to not only identify, but also gain a better understanding of new pluripotency markers to better characterize the human embryonic stem cell populations of interest. The novel hPlurES-1 Antibody is a monoclonal antibody that has been shown to identify a 65kD antigen that is expressed on the cell surface of human embryonic stem cells. This antibody recognizes a protein epitope that appears to be expressed within a subset of Oct-4-positive human embryonic stem cell populations, as indicated by double-staining analysis using flow cytometry and immunocytochemistry.\n\nApplications:\nSuitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nFlow Cytometry: 25ug/ml\nWestern Blot: 1.5ug/ml\nImmunocytochemistry: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

P4284

Size

100ug

Applications

FC, ICC, WB

Hosts

Mouse

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4. No preservatives added.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

Additional Info

Recognizes a cell surface epitope of a 65kD protein found to be expressed on pluripotent human embryonic stem cells.

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