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PMCA ATPase

Cat no: A4000-50A5


Supplier: United States Biological
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Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry (Fr) (paraffin-embedded sections). Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Catalogue number: A4000-50A5
Reactivities: Human, Rat, Canine, Chicken/Bird, Rabbit, Sheep
Hosts: Mouse
Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
Size: 100ul
Form: Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
P type: Mab
Isotype: IgG2a
Purity: Ascites
References: This product has been used in:Zaidi A & Michaelis ML Effects of reactive oxygen species on brain synaptic plasma membrane Ca(2+)-ATPase. Free Radic Biol Med 27:810-21 (1999). Filoteo AG et al. Plasma membrane Ca2+ pump in rat brain. Patterns of alternative splices seen by isoform-specific antibodies. J Biol Chem 272:23741-7 (1997). Borke JL et al. Plasma membrane calcium pump and 28-kDa calcium binding protein in cells of rat kidney distal tubules. Am J Physiol 257:F842-9 (1989). Liu BF et al. Consequences of functional expression of the plasma membrane Ca2+ pump isoform 1a. J Biol Chem 271:5536-44 (1996). Guerini D et al. Isolation and characterization of a stable Chinese hamster ovary cell line overexpressing the plasma membrane Ca(2+)-ATPase. J Biol Chem 270:14643-50 (1995). Schnitzer JE et al. Caveolae from luminal plasmalemma of rat lung endothelium: microdomains enriched in caveolin, Ca(2+)-ATPase, and inositol trisphosphate receptor. Proc Natl Acad Sci U S A 92:1759-63 (1995). Kuo TH et al. Phorbol ester induces both gene expression and phosphorylation of the plasma membrane Ca2+ pump. J Biol Chem 266:2520-5 (1991). Borke JL et al. Cellular and segmental distribution of Ca2(+)-pump epitopes in rat intestine. Pflugers Arch 417:120-2 (1990).
Additional info: This antibody detects all four known isoforms of the PMCA ATPase. Species Crossreactivity: Crossreacts with Human, Rabbit, Rat, canine, Sheep, Chicken and Primate.Not yet tested in other species.

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