Polyclonal Anti-ARG1

Price

200.00 USD

Size

100ug/vial

Reactivities

Mouse, Rat

Format

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

References

1. Cardoso, M. L., Martins, E., Vasconcelos, R., Vilarinho, L., Rocha, J. Identification of a novel R21X \nmutation in the liver-type arginase gene (ARG1) in four Portuguese patients with argininemia. Hum. \nMutat. 14: 355-356, 1999.\n2. Grody, W. W., Dodson, A., Klein, D., Kern, R. M., Bassand, P., Cederbaum, S. D. Molecular genetic \nstudy of human arginase deficiency. (Abstract) Am. J. Hum. Genet. 45 (suppl.): A191 only, 1989.\n3. Haraguchi, Y., Takiguchi, M., Amaya, Y., Kawamoto, S., Matsuda, I., Mori, M. Molecular cloning and \nnucleotide sequence of cDNA for human liver arginase. Proc. Nat. Acad. Sci. 84: 412-415, 1987.

Storage Temp

At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time.Avoid repeated freezing and thawing.

Scientific Background

ARG1(arginase, live) is a cytosolic enzyme and expressed predominantly in the liver as a component \nof the urea cycle. The isoform encoded by ARG1, referred to as the liver, or A-I, isoform, contributes \n98% of the arginase activity in liver but is also present in red cells. Using a rat liver ARG1 cDNA clone \nto probe a human liver cDNA library, Haraguchi et al. (1987) isolated and characterized a cDNA \ncorresponding to the ARG1 gene. The ARG1 gene is mapped on 6q23.2 and the arginase gene \ncontains 8 exons. By immunologic studies, 90% of the arginase in red blood cell and liver was \nprecipitated by the antibody, whereas only 50% of the arginase in kidney, brain, and the gastrointestinal \ntract reacted with it. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive \ndisorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have \nbeen found for this gene.